PhanerochaeteV1, Main, Exploration, bibRecord, 000756

Third-generation biosensor for lactose based on newly discovered cellobiose dehydrogenase.

Identifieur interne : 000756 ( Main/Exploration ); précédent : 000755; suivant : 000757

Third-generation biosensor for lactose based on newly discovered cellobiose dehydrogenase.

Auteurs : Leonard Stoica [Suède] ; Roland Ludwig ; Dietmar Haltrich ; Lo Gorton

Source :

Analytical chemistry [ 0003-2700 ] ; 2006.

RBID : pubmed:16408919

Descripteurs français

KwdFr :
- Animaux (MeSH), Basidiomycota (enzymologie), Bovins (MeSH), Carbohydrate dehydrogenases (composition chimique), Lactose (analyse), Lait (composition chimique), Techniques de biocapteur (méthodes), Électrochimie (MeSH).
MESH :
- analyse : Lactose.
- composition chimique : Carbohydrate dehydrogenases, Lait.
- enzymologie : Basidiomycota.
- méthodes : Techniques de biocapteur.
- Animaux, Bovins, Électrochimie.

English descriptors

KwdEn :
- Animals (MeSH), Basidiomycota (enzymology), Biosensing Techniques (methods), Carbohydrate Dehydrogenases (chemistry), Cattle (MeSH), Electrochemistry (MeSH), Lactose (analysis), Milk (chemistry).
MESH :
- chemical , analysis : Lactose.
- chemical , chemistry : Carbohydrate Dehydrogenases.
- chemistry : Milk.
- enzymology : Basidiomycota.
- methods : Biosensing Techniques.
- Animals, Cattle, Electrochemistry.

Abstract

The present paper describes the principle and characteristics of a biosensor for lactose based on a third-generation design involving cellobiose dehydrogenase. As resulted from a previous comparative study (submitted manuscript), the novelty of this lactose biosensor is based on highly efficient direct electron transfer between two newly discovered cellobiose dehydrogenases (CDH), from the white rot fungi Trametes villosa and Phanerochaete sordida, and a solid spectrographic graphite electrode. CDH was immobilized on the electrode surface (0.073 cm2) by simple physical adsorption, and the CDH-modified electrode was next inserted into a wall-jet amperometric cell connected on-line to a flow injection setup (0.5 mL x min(-1)). The P. sordida CDH-based lactose biosensor, proved to be the better one, has a detection limit for lactose of 1 microM, a sensitivity of 1100 microA x mM(-1) x cm(-2), a response time of 4 s (the time required to obtain the maximum peak current), and a linear range from 1 to 100 microM lactose (correlation coefficient 0.998). The simplicity of construction and analytical characteristics make this CDH-based lactose biosensor an excellent alternative to previous lactose biosensors reported in the literature or commercially available. The CDH-lactose sensor was used to quantify the content of lactose in pasteurized milk, buttermilk, and low-lactose milk, using the standard addition method. No effects of the samples matrixes were observed. The operational stability of the sensor was tested for 11 h by continuous injection of 100 microM lactose (290 injections). The final signal of the sensor was maintained at 98% of its initial signal, with a low standard deviation of 1.72 (RSD 2.41%).

DOI: 10.1021/ac050327o
PubMed: 16408919

Affiliations:

Suède

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Le document en format XML

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<author><name sortKey="Stoica, Leonard" sort="Stoica, Leonard" uniqKey="Stoica L" first="Leonard" last="Stoica">Leonard Stoica</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Analytical Chemistry, Lund University, P.O. Box 124, SE-221 00, Lund, Sweden. leonard.stoica@analykem.lu.se</nlm:affiliation>
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<author><name sortKey="Ludwig, Roland" sort="Ludwig, Roland" uniqKey="Ludwig R" first="Roland" last="Ludwig">Roland Ludwig</name>
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<term>Milk (chemistry)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term>
<term>Basidiomycota (enzymologie)</term>
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<term>Carbohydrate dehydrogenases (composition chimique)</term>
<term>Lactose (analyse)</term>
<term>Lait (composition chimique)</term>
<term>Techniques de biocapteur (méthodes)</term>
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<term>Lait</term>
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<front><div type="abstract" xml:lang="en">The present paper describes the principle and characteristics of a biosensor for lactose based on a third-generation design involving cellobiose dehydrogenase. As resulted from a previous comparative study (submitted manuscript), the novelty of this lactose biosensor is based on highly efficient direct electron transfer between two newly discovered cellobiose dehydrogenases (CDH), from the white rot fungi Trametes villosa and Phanerochaete sordida, and a solid spectrographic graphite electrode. CDH was immobilized on the electrode surface (0.073 cm2) by simple physical adsorption, and the CDH-modified electrode was next inserted into a wall-jet amperometric cell connected on-line to a flow injection setup (0.5 mL x min(-1)). The P. sordida CDH-based lactose biosensor, proved to be the better one, has a detection limit for lactose of 1 microM, a sensitivity of 1100 microA x mM(-1) x cm(-2), a response time of 4 s (the time required to obtain the maximum peak current), and a linear range from 1 to 100 microM lactose (correlation coefficient 0.998). The simplicity of construction and analytical characteristics make this CDH-based lactose biosensor an excellent alternative to previous lactose biosensors reported in the literature or commercially available. The CDH-lactose sensor was used to quantify the content of lactose in pasteurized milk, buttermilk, and low-lactose milk, using the standard addition method. No effects of the samples matrixes were observed. The operational stability of the sensor was tested for 11 h by continuous injection of 100 microM lactose (290 injections). The final signal of the sensor was maintained at 98% of its initial signal, with a low standard deviation of 1.72 (RSD 2.41%).</div>
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<Title>Analytical chemistry</Title>
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<ArticleTitle>Third-generation biosensor for lactose based on newly discovered cellobiose dehydrogenase.</ArticleTitle>
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<Abstract><AbstractText>The present paper describes the principle and characteristics of a biosensor for lactose based on a third-generation design involving cellobiose dehydrogenase. As resulted from a previous comparative study (submitted manuscript), the novelty of this lactose biosensor is based on highly efficient direct electron transfer between two newly discovered cellobiose dehydrogenases (CDH), from the white rot fungi Trametes villosa and Phanerochaete sordida, and a solid spectrographic graphite electrode. CDH was immobilized on the electrode surface (0.073 cm2) by simple physical adsorption, and the CDH-modified electrode was next inserted into a wall-jet amperometric cell connected on-line to a flow injection setup (0.5 mL x min(-1)). The P. sordida CDH-based lactose biosensor, proved to be the better one, has a detection limit for lactose of 1 microM, a sensitivity of 1100 microA x mM(-1) x cm(-2), a response time of 4 s (the time required to obtain the maximum peak current), and a linear range from 1 to 100 microM lactose (correlation coefficient 0.998). The simplicity of construction and analytical characteristics make this CDH-based lactose biosensor an excellent alternative to previous lactose biosensors reported in the literature or commercially available. The CDH-lactose sensor was used to quantify the content of lactose in pasteurized milk, buttermilk, and low-lactose milk, using the standard addition method. No effects of the samples matrixes were observed. The operational stability of the sensor was tested for 11 h by continuous injection of 100 microM lactose (290 injections). The final signal of the sensor was maintained at 98% of its initial signal, with a low standard deviation of 1.72 (RSD 2.41%).</AbstractText>
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Serveur d'exploration sur le phanerochaete

Third-generation biosensor for lactose based on newly discovered cellobiose dehydrogenase.

Third-generation biosensor for lactose based on newly discovered cellobiose dehydrogenase.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki